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Title   백혈병 세포 U - 937 에서 분화유도물질의 자극에 따른 세포표면 항원 및 암유전자 발현에 관한 연구 ( Expression of Cell Surface Antigens and Oncogene of Leukemic U - 937 Cells by Differentiation Inducers )
Publicationinfo   1992 Jan; 024(04): 480-493.
Key_word   U-937, c-myc, TPA, DHVD, DHEA, Glucocorticosteroid
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Abstract   The effect of I2-0-tetradecanoyl phorbo1-13-acetate(TPA), I, 25 dihydroxy-vitamin D,(DHVD,), dehydroepiandrosterone(DHEA), glucocorticosteroid(GCS) alone or in combination on the de- gree of cellular differentiation and the level of c-m expression was assessed in human myelomonocytic leukemic cell line U-937 by morphological observation, flow cytometry and Northern blot hybridization. 1) The TPA-treated U-937 cells showed to be gathered, to shape irregularly and to die, while either DHVD,-, DHEA-, or GCS-treated cells showed no change in the morphology. Among three combinations of TPA and other agents, the cells treated with combination of TPA and DHVD, were partly changed into long cells. 2) The mean fluorescence intensity of CD14 molecules on the cell surface and the percentage of the CD14+ cells were increased up to 78.66 and 61.2%, respectively in the cells treated with combination of TPA and DHVD, in comparison with the control cells(6.17, 2.9%). The mean flu-orescence intensity of CD15 moleules on the cell surface and the percentage of the CD15+ cells were decreased to 147.18 and 17.7%, respectively in the TPA-treated cells in compariosn with the control cells(292.09, 51.3%), whereas the expression levels of CD15 molecules on the cell surface treated with combined agents showed similar to the cells treated with TPA. 3) Poly(A+) mRNA was extracted from cells treated with various agents alone or in combina- tion for 3 or 18 hours. Northern blot analysis showed that the c-m expression of cells treated with TPA alone or combined agents for 3 hours decreased compared with control cells, while that of cells treated with combination of TPA and DHVD for 18 hours decreased significantly among various groups. 4) Control cells and cells treated with various agents showed the similar level of fluorescence intensity for antibody against c-m protein. These results indicated that the differentiation of U-937 cells related considerably to the increase of CD14 antigen expression and the decrease of c-m#w mRNA production.
저 자   오종석(Jong Suk Oh),이현희(Hyun Hee Lee),이현철(Hyun Hul Lee),신부안(Boo Ahn Shin),강인철(In Chol Kang),이정애(Jung Ae Rhee),정선식(Sun Sik Chung)